Material from the uterine hole may consist of tumefaction cells from ovarian, tubal or any other non- endometrial carcinoma, and it is essential to get information about their source, to validate the morphological analysis and also to determine the administration and treatment of clients, as plenty of dilemmas regarding ovarian and endometrial disease remains ambiguous. Study of aspirates and scrapes through the uterine cavity utilizing advanced molecular techniques, along with present examination methods, will help develop threat teams for uterine, tubal, ovarian as well as peritoneal tumors. The breakdown of literary works contains comparative qualities of various techniques and their combinations, which allow improving diagnostics of non-tumor lesions and endometrial tumors.The data of a complex immunoassay comparative study associated with content of soluble kinds of sPD-1, sPD-L1, sNKG2D, sNKG2DL1, sB7-H3 and sHLA-G into the bloodstream plasma of 75 patients with epithelial ovarian cancer and 20 healthy donors associated with the control team are presented. The diagnostic significance of the studied proteins ended up being determined. The research revealed that the profile of dissolvable immunity checkpoints differs when cancerous ovarian pathology takes place. There was clearly a statistically significant decline in this content of sPD-L1, sNKG2DL1, sB7-H3, and sHLA-G when you look at the bloodstream plasma of clients compared with the control group. Differences had been based in the content associated with studied markers with respect to the histological variety of tumors. Correlations between the soluble kinds of a few of the studied proteins are shown, suggesting the current presence of separate components of protected regulation in ovarian cancer, which may give an explanation for insufficient effectiveness for the existing immunotherapy because of this sort of cyst. The outcome gotten will undoubtedly facilitate the introduction of brand-new effective methods for the diagnostics and therapy of ovarian cancer.The presence of metabolic problem (MS) dramatically boosts the risk of building cardio diseases that cause chronic Multiplex immunoassay heart failure (CHF). The values of NT-proBNP, ST-2, and CRP markers and their mutual correlations were studied in 37 clients with chronic heart failure (CHF) without metabolic problem (MS) (group 1) and 37 customers with CHF with MS (group 2). The purpose of the analysis would be to figure out the features of their changes in clients with CHF difficult by MS, and also to position patients by assigning a rank value into the values of NTproBNP, ST2, and CRP concentrations. The average ST2 amount was 51±24 ng/ml in group 1 and 62±27 ng/ml in team 2. The average values of CRP in team 1 had been 23.1±5.3 mg/l, in group 2-33.0±4.4 mg/l (p less then 0.05). The NTproBNP degree ended up being 2413±1586 PG/ml and 2721±1635 PG/ml in groups 1 and 2, correspondingly. Correlations involving the values of NTproBNP and ST2, NTproBNP and CRP were demonstrated. In the group of CHF with MS, weighed against the group of CHF, there were much more customers with the most obvious pathological amounts of harm check details markers the number of patients with a broad ranking of 6-9 in the group of CHF with MS ended up being 59%, in the set of CHF without MS-38% (p less then 0.05). Of the 18 patients who died, 17 had been the type of who’d a complete ranking of 6 to 9, only one client just who died after hospitalization had a general ranking of 5. At the same time, among 22 customers that has improved CHF in the results of hospitalization, 18 patients had an overall total position from 0 to 5, as well as in 4 patients with this category, the clinical manifestations of CHF remained virtually unchanged. The outcomes of ranking the amount of the examined laboratory markers indicate they can be properly used as a predictor of varied results of CHF.Prolactin exists in several types including the monomeric biologically active form (23kDa) and a higher molecular weight form, bound most often to IgG, understood as macroprolactin (>100kDa). Macroprolactin does not have biological activity and is among the causes of false-positive results. In Russian Federation the most typical method for macroprolactin determination is PEG precipitation test. We had carried out a retrospective evaluation of 37 samples of customers with hyperprolactinemia (3 of all of them had been men). The mean age ended up being 30 [25;35] years. Prolactin degree ended up being Western Blotting measured by the immunoenzyme strategy with handbook PEG precipitation and TRACE. The mean values discovered by the immunoenzyme strategy with handbook PEG precipitation had been 461,6 [375,0;821,2] mU/l, by TRACE – 449,9 [357,2;749,2] mU/l. The amount of customers with normal prolactin levels was 30% (11) verified by two methods, large prolactin level at 46% (17). The prevalence of clinical symptoms of hyperprolactinemia was not differ depend the groups. The event of macroprolactinemia ended up being registered in 32% (12) of customers. In 8 persons with this team normal prolactin amount ended up being revealed as well as in 4 customers hyperprolactinemia ended up being discovered by TRACE. Measurements of prolactin levels by the TRACE strategy is useful for proper analysis in patients with equivocal outcomes obtained by traditional technique with PEG precipitation.